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blue s green qpcr kit  (Roche)


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    Roche blue s green qpcr kit
    Blue S Green Qpcr Kit, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lightcycler+480+qpcr+kit/LightCycler+480+System/pm41965332-430-33-28
    Average 99 stars, based on 6 article reviews
    blue s green qpcr kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Transcriptome-wide analysis of Arabidopsis DICER-LIKE1 RNA substrates
    Article Snippet: Total RNA was treated with DNaseI (Thermo Fisher Scientific) for 30 min at 37°C before reverse transcription with the Maxima First Strand cDNA Synthesis Kit (Thermo Fisher Scientific). sqPCRs were performed using the DreamTaq DNA polymerase (Thermo Fiser Scientific) and the indicated primers ( ). .. Real-time qPCRs were performed on a LightCycler480 II (Roche) with the KAPA SYBR ® FAST for LightCycler ® 480 qPCR Kit (KAPA Biosystems) and the indicated primers , following the PCR program recommended with the KAPA SYBR FAST qPCR mix. ..

    Article Title: Nucleo-cytosolic Shuttling of ARGONAUTE1 Prompts a Revised Model of the Plant MicroRNA Pathway.
    Article Snippet: Total RNA was treated with DNaseI (Thermo Fisher Scientific) for 30 min at 37 C before reverse transcription with the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific). .. Real-time qPCR was performed on a LightCycler480 II (Roche) with the KAPA SYBR FAST for LightCycler 480 qPCR Kit (KAPA Biosystems) and gene-specific primers (Table S3). ..

    Polymerase Chain Reaction:

    Article Title: Transcriptome-wide analysis of Arabidopsis DICER-LIKE1 RNA substrates
    Article Snippet: Total RNA was treated with DNaseI (Thermo Fisher Scientific) for 30 min at 37°C before reverse transcription with the Maxima First Strand cDNA Synthesis Kit (Thermo Fisher Scientific). sqPCRs were performed using the DreamTaq DNA polymerase (Thermo Fiser Scientific) and the indicated primers ( ). .. Real-time qPCRs were performed on a LightCycler480 II (Roche) with the KAPA SYBR ® FAST for LightCycler ® 480 qPCR Kit (KAPA Biosystems) and the indicated primers , following the PCR program recommended with the KAPA SYBR FAST qPCR mix. ..



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    Neuroactive and carbohydrate metabolic pathways in flight muscles under extreme hypoxia in B. terrestris (A) Transcriptome validation of randomly selected key genes (e.g., 5-HT 1A , etc.) in the neuroactive ligand-receptor interaction pathway using quantitative reverse-transcription PCR (RT-qPCR). n = 3 biological replicates of three bumblebees for all assays. (B) Expression profiles of genes involved in sugar metabolism pathways. Heatmap showing log 2 (fold change) in response to hypoxic exposure. N1–3 represent the control group, and H1–3 represent the hypoxic treatment group. (C) Measurement of glucose, glycogen, and trehalose concentrations involved in carbohydrate metabolism. (D) Quantification of pyruvate and ATP levels. (E) Measurement of the GSH/GSSG ratio as an indicator of cellular redox status. n = 3 biological replicates of three bumblebees for all assays. Data are presented as means ± SEM. Statistical significance between groups was determined using Student’s t tests. (F) Proposed model of hypoxia-regulated biological processes in flight muscles, integrating transcriptomic and targeted metabolite quantification.

    Journal: iScience

    Article Title: Oxygen-dependent regulation of serotonin receptor 5-HT 1A in hypoxic response and flight performance in bumblebees, Bombus terrestris

    doi: 10.1016/j.isci.2025.114211

    Figure Lengend Snippet: Neuroactive and carbohydrate metabolic pathways in flight muscles under extreme hypoxia in B. terrestris (A) Transcriptome validation of randomly selected key genes (e.g., 5-HT 1A , etc.) in the neuroactive ligand-receptor interaction pathway using quantitative reverse-transcription PCR (RT-qPCR). n = 3 biological replicates of three bumblebees for all assays. (B) Expression profiles of genes involved in sugar metabolism pathways. Heatmap showing log 2 (fold change) in response to hypoxic exposure. N1–3 represent the control group, and H1–3 represent the hypoxic treatment group. (C) Measurement of glucose, glycogen, and trehalose concentrations involved in carbohydrate metabolism. (D) Quantification of pyruvate and ATP levels. (E) Measurement of the GSH/GSSG ratio as an indicator of cellular redox status. n = 3 biological replicates of three bumblebees for all assays. Data are presented as means ± SEM. Statistical significance between groups was determined using Student’s t tests. (F) Proposed model of hypoxia-regulated biological processes in flight muscles, integrating transcriptomic and targeted metabolite quantification.

    Article Snippet: SYBR Green qPCR Kit , Roche, USA , Cat# 04707516001.

    Techniques: Muscles, Biomarker Discovery, Reverse Transcription, Quantitative RT-PCR, Expressing, Control